In the tag question "Clean your room, will you?", the correct tag question to use would be "won't you?". The tag question should be in the opposite form of the main clause, which in this case is a positive imperative sentence ("Clean your room"). Therefore, the appropriate tag question would be a negative one.
So, the correct tag question would be "Clean your room, won't you?" This tag question seeks confirmation or agreement, implying a request for the person to clean their room.